Anti -PCNA Rabbit pAb

價格：在線咨詢

貨號：

DRF10150

產品規(guī)格：

100 μL

Applications：

WB/IHC/IF

Species：

H/M/R,H/M/R,H/M/R

品牌：

萬物

購買數(shù)量：

立即詢價在線咨詢 400-1016-218

產品詳情操作說明注意事項

Product Information

Description	PCNA rabbit polyclonal
Protein full name	Proliferating cell nuclear antigen
Synonyms	PCNA, ATLD2, proliferating cell nuclear antigen
Immunogen	KLH conjugated Synthetic peptide corresponding to Mouse PCNA
Isotype	IgG
Subcellular location	Nucleus
Predicted MW.	29 kDa
Observed MW.	36 kDa
Purity	Affinity purification
Uniprot ID	P12004, P17918, P04961

Applications

applications	species	dilution	Positive tissue
WB	Human, Mouse, Rat	1: 1000-1: 2000	Hela, Jurkat, Raw264.7, MCF7, 293, NIH/3T3, A431, HepG2, SW480, B16, C6, HCT116
IHC	Human, Mouse, Rat	1: 300-1: 1000	lung cancer, liver cancer
IF	Human, Mouse, Rat	1: 200-1: 1000	ovarian cancer, small intestine cancer, testis

Background

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.

Images

	Western blot analysis of PCNA (GB11010) at dilution of 1: 2000 Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: Raw264.7 cell lysate Lane 4: MCF7 cell lysate Lane 5: 293 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: A431 cell lysate Lane 8: HepG2 cell lysate
	Western blot analysis of PCNA (GB11010) at dilution of 1: 2000 Lane 1: HeLa cell lysate Lane 2: SW480 cell lysate Lane 3: B16 cell lysate Lane 4: C6 cell lysate Lane 5: 7721 cell lysate Lane 6: HCT116 cell lysate
	Immunohistochemistry analysis of paraffin-embedded human lung cancer using PCNA (GB11010) at dilution of 1: 500
	Immunohistochemistry analysis of paraffin-embedded human liver cancer using PCNA (GB11010) at dilution of 1: 500
	Immunofluorescent analysis of paraformaldehyde-fixed human ovarian cancer using PCNA (GB11010) at dilution of 1: 500
	Immunofluorescent analysis of paraformaldehyde-fixed human small intestine cancer using PCNA (GB11010) at dilution of 1: 500
	Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using PCNA (GB11010) at dilution of 1: 500
	Immunofluorescent analysis of paraformaldehyde-fixed rat testis using PCNA (GB11010) at dilution of 1: 500

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

凡合肥萬物生物科技有限公司銷售的產品均有嚴格的質量保證。如發(fā)現(xiàn)產品存在包裝規(guī)格疑問，請于收貨之日起七日內向我公司總部或當?shù)剞k事處提出，并請保證該產品，以備本公司進行核對檢驗。如果發(fā)現(xiàn)有質量問題，請于收貨后一月內保留產品50%以上的量，以便本公司回收產品進行質檢，確認產品質量。如逾期，則視為已經對本公司產品進行驗收。聲明：如發(fā)生產品索賠事宜，本公司僅在產品價格范圍內酌情賠償，恕不接受超出產品價格范圍之賠償。客戶在使用過程中有任何技術問題可以撥打技術售后服務電話我們隨時給予解答。

合肥萬物生物科技有限公司

客服熱線：400-1016-218

QQ：355185756

地址：安徽省合肥市高新區(qū)黃山路602號合肥國家大學科技園A401室