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Anti -PCNA Rabbit pAb

價格: 在線咨詢
貨號:
DRF10151
產(chǎn)品規(guī)格:
100 μL
Applications:
WB/IHC/IF
Species:
H,H/M,H/M
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

PCNA rabbit polyclonal

Protein full name

Proliferating cell nuclear antigen

Synonyms

PCNA, ATLD2, proliferating cell nuclear antige

Immunogen

Recombinant protein corresponding to Mouse PCNA

Isotype

IgG

Purity

Affinity purification

Predicted MW.

29 kDa

Observed MW.

36 kDa

Subcellular location

Nucleus

Uniprot ID

Q12004, P17918, P04961



Applications


applications

species

dilution

Positive tissue

WB

Human

1: 500-1: 2000

HeLa, HepG2, MCF7, liver cancer

IHC

Human,

Mouse

1: 1000-1: 2000

lung cancer, heart

IF

Human,

Mouse

1: 400-1: 2000

lung cancer, testis, ovary cancer



Background



Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.




Images



Western blot analysis of PCNA (GB11010-1) at dilution of 1: 1000

Lane 1: HeLa cell lysate

Lane 2: MCF-7 cell lysate

Lane 3: 293T cell lysate

Lane 4: NIH/3T3 cell lysate

Lane 5: PC-12 cell lysate

Lane 6: Mouse testis tissue lysate

Lane 7: Mouse colon tissue lysate

Lane 8: Rat testis tissue lysate

Lane 9: Rat colon tissue lysate

Immunohistochemistry analysis of paraffin-embedded human lung cancer using PCNA (GB11010-1) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded mouse heart using PCNA (GB11010-1) at dilution of 1: 2000

Immunofluorescent analysis of paraformaldehyde-fixed human lung cancer using PCNA (GB11010-1) at dilution of 1: 2000


Immunofluorescent analysis of paraformaldehyde-fixed human ovary cancer using PCNA (GB11010-1) at dilution of 1: 2000


Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using 

PCNA (GB11010-1) at dilution of 1: 2000





Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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