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Product Information
Description |
PCNA rabbit polyclonal |
Protein full name |
Proliferating cell nuclear antigen |
Synonyms |
PCNA, ATLD2, proliferating cell nuclear antige |
Immunogen |
Recombinant protein corresponding to Mouse PCNA |
Isotype |
IgG |
Purity |
Affinity purification |
Predicted MW. |
29 kDa |
Observed MW. |
36 kDa |
Subcellular location |
Nucleus |
Uniprot ID |
Applications
applications |
species |
dilution |
Positive tissue |
WB |
Human |
1: 500-1: 2000 |
HeLa, HepG2, MCF7, liver cancer |
IHC |
Human, Mouse |
1: 1000-1: 2000 |
lung cancer, heart |
IF |
Human, Mouse |
1: 400-1: 2000 |
lung cancer, testis, ovary cancer |
Background
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.
Images
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Western blot analysis of PCNA (GB11010-1) at dilution of 1: 1000 Lane 1: HeLa cell lysate Lane 2: MCF-7 cell lysate Lane 3: 293T cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Mouse colon tissue lysate Lane 8: Rat testis tissue lysate Lane 9: Rat colon tissue lysate |
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Immunohistochemistry analysis of paraffin-embedded human lung cancer using PCNA (GB11010-1) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded mouse heart using PCNA (GB11010-1) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed human lung cancer using PCNA (GB11010-1) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed human ovary cancer using PCNA (GB11010-1) at dilution of 1: 2000
|
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Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using PCNA (GB11010-1) at dilution of 1: 2000
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Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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