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Product Information
Description |
Ki67 rabbit polyclonal |
Protein full name |
Proliferation marker protein Ki-67 |
Synonyms |
MKI67, marker of proliferation Ki-67, KIA, MIB-, MIB-1, PPP1R105 |
Immunogen | Recombinant protein corresponding to Mouse Ki67 |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. | 359KDa |
Observed MW. |
359KDa |
Subcellular location |
Nucleus,nucleolus |
Uniprot ID | E9PVX6 |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Mouse |
1:300-1:600 |
Hela,HepG2,A431,liver,stomach,colon |
Background
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity).
Images
|
Western blot analysis of Ki67 (GB11030) at dilution of 1:600. Lane 1:Hela cell lysate Lane 2:HepG2 cell lysate Lane 3:A431 cell lysate Lane 4:Human liver cancer tissue lysate Lane 5:Human stomach cancer tissue lysate Lane 6:Mouse colon tissue lysate
|
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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