Anti -HIF-1 alpha Rabbit pAb

價格：在線咨詢

貨號：

DRF10168

產(chǎn)品規(guī)格：

100 μL

Applications：

Species：

H/M/R

品牌：

萬物

購買數(shù)量：

立即詢價在線咨詢 400-1016-218

產(chǎn)品詳情操作說明注意事項

Product Information

Description	HIF-1 alpha rabbit polyclonal
Protein full name	Hypoxia-inducible factor 1-alpha
Synonyms	HIF1A, HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, hypoxia inducible factor 1 alpha subunit
Immunogen	KLH conjugated Synthetic peptide corresponding to Mouse HIF-1α
Isotype	IgG
Purity	Affinity purification
Predicted MW.	93/120KDa
Observed MW.	93KDa
Uniprot ID	Q16665,Q61221,O35800

Applications

Applications	Species	Dilution	Positive Tissue
WB	Human, Mouse, Rat	1:500-1:1000	Hela,HepG2,HCT116,lung,brain

Background

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

Images

Western blot analysis of HIF-1α(GB11031) at dilution of 1:1000.

Lane 1:Hela cell lysate

Lane 2:Hela cell lysate (treated with COCL2)

Lane 3:HepG2 cell lysate

Lane 4:HCT116 cell lysate

Lane 5:Mouse lung tissue lysate

Lane 6:Mouse brain tissue lysate

Lane 7:Rat lung tissue lysate

Lane 8:Rat brain tissue lysate

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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