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Anti -GFAP Rabbit pAb

價格: 在線咨詢
貨號:
DRF101109
產品規(guī)格:
100 μL
Applications:
WB/IHC/IF
Species:
H/M/R,M/R,M/R
購買數量:
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產品詳情 操作說明 注意事項

Product Information


Description

GFAP rabbit polyclonal

Protein full name

Glial fibrillary acidic protein

Synonyms

GFAP, ALXDRD, glial fibrillary acidic protein, Astrocyte, Intermediate filament protein

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse GFAP 

Isotype

IgG

Predicted MW.

49 kDa

Observed MW.

49 kDa

Purity

Affinity purification

Subcellular location

Cytoplasm

Uniprot ID

P14136P03995P47819



Applications


applications

species

dilution

Positive tissue

WB

Human, Mouse, Rat

1: 1000-1: 2000

brain, cerebellum, cortex

IHC

Mouse, Rat

1: 1000-1: 2000

brain, cerebellum

IF

Mouse, Rat

1: 500-1: 3000

brain, hippocampus



Background


Glial fibrillary acidic protein is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells.GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. It is closely related to its non-epithelial family members, vimentin,desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton.




Images



Western blot analysis of GFAP (GB11096) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded mouse brain using 

GFAP (GB11096) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded rat brain using 

GFAP (GB11096) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded rat cerebellum using 

GFAP (GB11096) at dilution of 1: 2000


Immunofluorescent analysis of paraformaldehyde-fixed mouse brain using 

GFAP (GB11096) at dilution of 1: 1000


Immunofluorescent analysis of paraformaldehyde-fixed rat brain using 

GFAP (GB11096) at dilution of 1: 1000


Immunofluorescent analysis of paraformaldehyde-fixed rat hippocampus using Neun (GB11138) (green) + IBA-1 (GB12105) (red) + MBP (GB12226) (spred) +GFAP (GB11096) (yellow) and DAPI in blue at dilution of 1: 1000




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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