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Anti -GRP78 BiP Rabbit pAb

價格: 在線咨詢
貨號:
DRF101113
產(chǎn)品規(guī)格:
100 μL
Applications:
WB/IHC/IF
Species:
H/M/R,H/M/R,H/M/R
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

GRP78 BiP rabbit polyclonal

Protein full name

78 kDa glucose-regulated protein

Synonyms

HSPA5, BIP, GRP78, HEL-S-89n, MIF2, Binding immunoglobulin protein, heat shock protein family A (Hsp70) member 5

Immunogen

Recombinant protein corresponding to  Mouse GRP78

Isotype

IgG

Predicted MW.

78 kDa

Observed MW.

78 kDa

Purity

Affinity purification

Subcellular location

Endoplasmic reticulum lumen, Melanosome, Cytoplasm

Uniprot ID

P11021, P20029, P06761




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-

1: 1000

lung, kidney

IHC

Human, Mouse, Rat

1: 1000-1: 2000

kidney, brain

IF

Human, Mouse, Rat

1: 1000-1: 2000

kidney, brain




Background


Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.




Images


圖片 1.png

Western blot analysis of GRP78 (GB11098-1) at dilution of 1: 1000.

Lane 1: HeLa cell lysate

Lane 2: HepG2 cell lysate

Lane 3: MCF7 cell lysate

Lane 4: A549 cell lysate

Lane 5: 786-0 cell lysate

Lane 6: Mouse lung tissue lysate

Lane 7: Mouse kidney tissue lysate

Lane 8: Rat lung tissue lysate

Lane 9: Rat kidney tissue lysate


blob.png

Immunohistochemistry analysis of paraffin-embedded mouse brain using GRP78 (GB11098-1) at dilution of 1: 2000


blob.png

Immunohistochemistry analysis of paraffin-embedded rat brain using GRP78 (GB11098-1) at dilution of 1: 2000




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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