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Anti -Collagen V Rabbit pAb

價格: 在線咨詢
貨號:
DRF101127
產(chǎn)品規(guī)格:
100 μL
Applications:
WB/IHC/IF
Species:
M/R,M/R,M/R
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

Collagen V rabbit polyclonal

Protein full name

Collagen alpha-1(V) chain

Synonyms

Col5a1, Collagen Type V, Alpha 1 type V collagen, Col5A2, Col5A3, Collagen alpha 2(V) chain, Collagen type V alpha 1

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Collagen V

Isotype

IgG

Purity

Affinity purification

Subcellular location

Extracellular region or secreted

Predicted MW.

184 kDa

Observed MW.

184 kDa

Uniprot ID

O88207, Q9JI03




Applications


Applications

Species

Dilution

Positive Tissue

WB

Mouse, Rat

1: 500-1: 1000

liver fibrosis

IHC

Mouse, Rat

1: 1500-1: 3000

skin, heart

IF

Mouse, Rat

1: 750-1: 1500

skin, heart




Background


COL5A1 encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. COL5A1 product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II.




Images


image.png

Western blot analysis of Collagen V (GB111012) at dilution of 1: 1000

Lane 1: Mouse liver fibrosis tissue lysate

image.png

Immunohistochemistry analysis of paraffin-embedded mouse skin using Collagen V (GB111012) at dilution of 1: 1800

image.png

Immunohistochemistry analysis of paraffin-embedded mouse heart using Collagen V (GB111012) at dilution of 1: 1800




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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