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Product Information
Description |
Nucleophosmin rabbit polyclonal |
Protein full name |
Nucleophosmin |
Synonyms |
NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, Npm1 |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Nucleophosmin |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
|
Predicted MW. |
33 kDa |
Observed MW. |
40 kDa |
Uniprot ID |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
spleen, testis |
IHC |
Human, Mouse, Rat |
1: 1000-1: 3000 |
lung cancer, liver cancer, colon cancer, testis, colon, lymph, ovary, spleen |
IF |
Human, Mouse, Rat |
1: 500-1: 2500 |
lung cancer, liver cancer, colon cancer, testis, colon, lymph, ovary, spleen |
Background
Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation and regulation of tumor suppressors p53/TP53 and ARF.
Stimulates APEX1endonuclease activity on apurinic/apyrimidinic double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules.
Images
|
Western blot analysis of Nucleophosmin (GB111043) at dilution of 1: 1000 Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: 293T cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC12 cell lysate Lane 6: Mouse spleen tissue lysate Lane 7: Mouse testis tissue lysate Lane 8: Rat spleen tissue lysate Lane 9: Rat testis tissue lysate |
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Immunohistochemistry analysis of paraffin-embedded human lung cancer using Nucleophosmin (GB111043) at dilution of 1:6000 |
|
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Nucleophosmin (GB111043) at dilution of 1:6000 |
|
Immunohistochemistry analysis of paraffin-embedded human colon cancer using Nucleophosmin (GB111043) at dilution of 1:6000 |
|
Immunohistochemistry analysis of paraffin-embedded mouse testis using Nucleophosmin (GB111043) at dilution of 1:6000 |
|
Immunohistochemistry analysis of paraffin-embedded mouse colon using Nucleophosmin (GB111043) at dilution of 1:6000 |
|
Immunohistochemistry analysis of paraffin-embedded mouse lymph using Nucleophosmin (GB111043) at dilution of 1:6000 |
|
Immunohistochemistry analysis of paraffin-embedded mouse ovary using Nucleophosmin (GB111043) at dilution of 1:6000 |
|
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Nucleophosmin (GB111043) at dilution of 1:6000 |
|
Immunohistochemistry analysis of paraffin-embedded rat testis using Nucleophosmin (GB111043) at dilution of 1:6000 |
|
Immunohistochemistry analysis of paraffin-embedded rat colon using Nucleophosmin (GB111043) at dilution of 1:6000 |
|
Immunohistochemistry analysis of paraffin-embedded mouse lymph using Nucleophosmin (GB111043) at dilution of 1:6000 |
|
Immunohistochemistry analysis of paraffin-embedded rat ovary using Nucleophosmin (GB111043) at dilution of 1:6000 |
|
Immunohistochemistry analysis of paraffin-embedded rat spleen using Nucleophosmin (GB111043) at dilution of 1:6000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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