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Anti -Nucleophosmin Rabbit pAb

價(jià)格: 在線咨詢
貨號(hào):
DRF101161
產(chǎn)品規(guī)格:
100 μL
Applications:
Species:
購(gòu)買數(shù)量:
產(chǎn)品詳情 操作說明 注意事項(xiàng)

Product Information


Description

Nucleophosmin rabbit polyclonal

Protein full name

Nucleophosmin

Synonyms

NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, Npm1

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Nucleophosmin

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Predicted MW.

33 kDa

Observed MW.

40 kDa

Uniprot ID

P06748, Q61937, P13084




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

spleen, testis

IHC

Human, Mouse, Rat

1: 1000-1: 3000

lung cancer, liver cancer, colon cancer, testis, colon, lymph, ovary, spleen

IF

Human, Mouse, Rat

1: 500-1: 2500

lung cancer, liver cancer, colon cancer, testis, colon, lymph, ovary, spleen




Background


Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation and regulation of tumor suppressors p53/TP53 and ARF.

Stimulates APEX1endonuclease activity on apurinic/apyrimidinic double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules.




Images


image.png

Western blot analysis of Nucleophosmin (GB111043) at dilution of 1: 1000

Lane 1: HepG2 cell lysate

Lane 2: MCF7 cell lysate

Lane 3: 293T cell lysate

Lane 4: NIH/3T3 cell lysate

Lane 5: PC12 cell lysate

Lane 6: Mouse spleen tissue lysate

Lane 7: Mouse testis tissue lysate

Lane 8: Rat spleen tissue lysate

Lane 9: Rat testis tissue lysate

image.png

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Nucleophosmin (GB111043) at dilution of 1:6000

image.png

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Nucleophosmin (GB111043) at dilution of 1:6000

image.png

Immunohistochemistry analysis of paraffin-embedded human colon cancer using Nucleophosmin (GB111043) at dilution of 1:6000

image.png

Immunohistochemistry analysis of paraffin-embedded mouse testis using Nucleophosmin (GB111043) at dilution of 1:6000

image.png

Immunohistochemistry analysis of paraffin-embedded mouse colon using Nucleophosmin (GB111043) at dilution of 1:6000

image.png

Immunohistochemistry analysis of paraffin-embedded mouse lymph using Nucleophosmin (GB111043) at dilution of 1:6000

image.png

Immunohistochemistry analysis of paraffin-embedded mouse ovary using Nucleophosmin (GB111043) at dilution of 1:6000

image.png

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Nucleophosmin (GB111043) at dilution of 1:6000

image.png

Immunohistochemistry analysis of paraffin-embedded rat testis using Nucleophosmin (GB111043) at dilution of 1:6000

image.png

Immunohistochemistry analysis of paraffin-embedded rat colon using Nucleophosmin (GB111043) at dilution of 1:6000

image.png

Immunohistochemistry analysis of paraffin-embedded mouse lymph using Nucleophosmin (GB111043) at dilution of 1:6000

image.png

Immunohistochemistry analysis of paraffin-embedded rat ovary using Nucleophosmin (GB111043) at dilution of 1:6000

image.png

Immunohistochemistry analysis of paraffin-embedded rat spleen using Nucleophosmin (GB111043) at dilution of 1:6000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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