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Anti -Tollip Rabbit pAb

價格: 在線咨詢
貨號:
DRF101215
產(chǎn)品規(guī)格:
100 μL
Applications:
WB/IHC/IF
Species:
M/R,H/M/R,H/M/R
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

Tollip rabbit polyclonal

Protein full name

Toll-interacting protein

Synonyms

TOLLIP, IL-1RAcPIP, toll interacting protein

Immunogen

Recombinant protein corresponding to

Mouse Tollip

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW.

30 kDa

Observed MW.

30 kDa

Uniprot ID

Q9H0E2, Q9QZ06




Applications


Applications

Species

Dilution

Positive Tissue

WB

Mouse, Rat

1 :500-1: 1000

brain, spleen, thymus

IHC

Human, Mouse, Rat

1: 1000-1: 2000

liver cancer, spleen, brain, kidney, lung

IF

Human, Mouse, Rat

1: 500-1: 1000

liver cancer, spleen, brain, kidney, lung




Background


The Tollip is the component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates




Images


Western blot analysis of NCR1 (GB111102) at dilution of 1: 1000
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse thymus tissue lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse spleen tissue lysate
Lane 6: Mouse thymus tissue lysate

圖片3.png

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Tollip (GB111093) at dilution of 1: 2000

圖片4.png

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Tollip (GB111093) at dilution of 1: 2000

圖片5.png

Immunohistochemistry analysis of paraffin-embedded rat kidney using Tollip (GB111093) at dilution of 1: 2000

圖片6.png

Immunohistochemistry analysis of paraffin-embedded rat brain using Tollip (GB111093) at dilution of 1: 2000

圖片7.png

Immunohistochemistry analysis of paraffin-embedded rat lung using Tollip (GB111093) at dilution of 1: 2000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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