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Product Information
Description |
eRF1 rabbit polyclonal |
Protein full name |
Eukaryotic peptide chain release factor subunit 1 |
Synonyms |
Eukaryotic release factor 1, eRF1, Etf1, ERF, RF1 |
Immunogen |
Recombinant protein corresponding to Mouse eRF1 |
Isotype |
IgG |
Purity |
Affinity purification |
Predicted MW. |
49 kDa |
Observed MW. |
50 kDa |
Subcellular location |
|
Uniprot ID |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
liver, kidney, testis |
IHC |
Rat |
1: 1500-1: 4000 |
brain, testis |
IF |
Rat |
1: 750-1: 1500 |
brain, testis |
Background
This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in coding and non-coding transcript variants. Pseudogenes of this gene are found on chromosomes 5, 7 and X.
Images
Western blot analysis of eRF1 (GB111098) at dilution of 1: 1000 |
|
Immunohistochemistry analysis of paraffin-embedded rat brain using eRF1 (GB111098) at dilution of 1: 2400 |
|
Immunohistochemistry analysis of paraffin-embedded rat testis using eRF1 (GB111098) at dilution of 1: 2400 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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