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Anti -eRF1 Rabbit pAb

價(jià)格: 在線咨詢
貨號(hào):
DRF101220
產(chǎn)品規(guī)格:
100 μL
Applications:
WB/IHC/IF
Species:
H/M/R,R,R
購(gòu)買數(shù)量:
產(chǎn)品詳情 操作說明 注意事項(xiàng)

Product Information


Description

eRF1 rabbit polyclonal

Protein full name

Eukaryotic peptide chain release factor subunit 1

Synonyms

Eukaryotic release factor 1, eRF1, Etf1, ERF, RF1

Immunogen

Recombinant protein corresponding to Mouse eRF1

Isotype

IgG

Purity

Affinity purification

Predicted MW.

49 kDa

Observed MW.

50 kDa

Subcellular location

Cytoplasm

Uniprot ID

P62495, Q8BWY3, Q5U2Q7




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

liver, kidney, testis

IHC

Rat

1: 1500-1: 4000

brain, testis

IF

Rat

1: 750-1: 1500

brain, testis




Background


This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in coding and non-coding transcript variants. Pseudogenes of this gene are found on chromosomes 5, 7 and X.




Images


Western blot analysis of eRF1 (GB111098) at dilution of 1: 1000
Lane 1: A431 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Mouse liver tissue lysate
Lane 5: Mouse kidney tissue lysate
Lane 6: Mouse testis tissue lysate
Lane 7: Rat kidney tissue lysate
Lane 8: Rat testis tissue lysate

Immunohistochemistry analysis of paraffin-embedded rat brain using eRF1 (GB111098) at dilution of 1: 2400

Immunohistochemistry analysis of paraffin-embedded rat testis using eRF1 (GB111098) at dilution of 1: 2400




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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