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Anti -Cyclin A2 Rabbit pAb

價格: 在線咨詢
貨號:
DRF101256
產(chǎn)品規(guī)格:
100 μL
Applications:
WB/IHC/IF
Species:
H/M,H/M/R,H/M/R
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

Cyclin A2 rabbit polyclonal

Protein full name

Cyclin-A2

Synonyms

Cyclin-A, Cyclin A, CCNA2, CCN1, CCNA

Immunogen

Recombinant protein corresponding to Human Cyclin A2

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Predicted MW.

49 kDa

Observed MW.

55 kDa

Uniprot ID

P20248, P51943, G3V802




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse

1: 500-1: 1000

lymph nodes, spleen

IHC

Human, Mouse, Rat

1: 600-1: 1200

lymph nodes,

brain, testis

IF

Human, Mouse, Rat

1: 250-1: 1000

lung cancer, kidney




Background


Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.





Images


Western blot analysis of Cyclin A2 (GB111132)

at dilution of 1: 1000

Lane 1: HCT116 cell lysate

Lane 2: Jurkat cell lysate

Lane 3: HT29 cell lysate

Lane 4: K562 cell lysate

Lane 5: SW480 cell lysate

Lane 6: Mouse lymph nodes tissue lysate

Lane 7: Mouse spleen tissue lysate

Immunohistochemistry analysis of paraffin-embedded using human lymph nodes Cyclin A2 (GB111132) at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded using mouse brain Cyclin A2 (GB111132) at dilution of 1: 600

Immunohistochemistry analysis of paraffin-embedded using rat testis Cyclin A2 (GB111132) at dilution of 1: 600

Immunofluorescent analysis of paraffin-embedded using human lung cancer Cyclin A2 (GB111132) at dilution of 1: 500

Immunofluorescent analysis of paraffin-embedded using mouse kidney Cyclin A2 (GB111132) at dilution of 1: 500




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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