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Anti -Ki67 Rabbit pAb

價(jià)格: 在線咨詢
貨號(hào):
DRF101266
產(chǎn)品規(guī)格:
100 μL
Applications:
IHC/IF
Species:
M/R,M/R
購(gòu)買(mǎi)數(shù)量:
產(chǎn)品詳情 操作說(shuō)明 注意事項(xiàng)

Product Information


Description

Ki67 rabbit polyclonal

Protein full name

Proliferation marker protein Ki-67

Synonyms

MKI67, marker of proliferation Ki-67, KIA,

MIB-1, PPP1R105

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse Ki67

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Uniprot ID

E9PVX6



Applications


applications

species

dilution

Positive tissue

IHC

Mouse, Rat

1: 500-1: 1000

colon, placenta

IF

Mouse, Rat

1: 600-1: 1200

testis, colon




Background


Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity).




Images


Immunohistochemistry analysis of paraffin-embedded mouse placenta using 

Ki67 (GB111141) at dilution of 1: 500

Immunohistochemistry analysis of paraffin-embedded mouse colon using 

Ki67 (GB111141) at dilution of 1: 500

Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using Ki67 (GB111141) at dilution of 1: 1200
Immunofluorescent analysis of paraformaldehyde-fixed rat colon using Ki67 (GB111141) at dilution of 1: 1200




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


Publications:



Title

Journal

Pubmed ID

Application

1

p38/JNK Is Required for the Proliferation and Phenotype Changes of Vascular Smooth Muscle Cells Induced by L3MBTL4 in Essential Hypertension.

International Journal of Hypertension

33381308

IHC

2

Simvastatin re-sensitizes hepatocellular carcinoma cells to sorafenib by inhibiting HIF-1α/PPAR-γ/PKM2-mediated glycolysis.

Journal of Experimental & Clinical Cancer Research : CR

32000827

IHC

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