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Product Information
Description |
Ki67 rabbit polyclonal |
Protein full name |
Proliferation marker protein Ki-67 |
Synonyms |
MKI67, marker of proliferation Ki-67, KIA, MIB-1, PPP1R105 |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Ki67 |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Nucleus |
Uniprot ID |
E9PVX6 |
Applications
applications |
species |
dilution |
Positive tissue |
IHC |
Mouse, Rat |
1: 500-1: 1000 |
colon, placenta |
IF |
Mouse, Rat |
1: 600-1: 1200 |
testis, colon |
Background
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity).
Images
Immunohistochemistry analysis of paraffin-embedded mouse placenta using Ki67 (GB111141) at dilution of 1: 500 |
|
Immunohistochemistry analysis of paraffin-embedded mouse colon using Ki67 (GB111141) at dilution of 1: 500 |
|
Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using Ki67 (GB111141) at dilution of 1: 1200 | |
Immunofluorescent analysis of paraformaldehyde-fixed rat colon using Ki67 (GB111141) at dilution of 1: 1200 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
Publications:
|
Title |
Journal |
Pubmed ID |
Application |
1 |
p38/JNK Is Required for the Proliferation and Phenotype Changes of Vascular Smooth Muscle Cells Induced by L3MBTL4 in Essential Hypertension. |
International Journal of Hypertension |
33381308 |
IHC |
2 |
Simvastatin re-sensitizes hepatocellular carcinoma cells to sorafenib by inhibiting HIF-1α/PPAR-γ/PKM2-mediated glycolysis. |
Journal of Experimental & Clinical Cancer Research : CR |
32000827 |
IHC |
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