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Anti -PABP Rabbit pAb

價格: 在線咨詢
貨號:
DRF101289
產品規(guī)格:
100 μL
Applications:
WB | IHC | IF
Species:
H,M,R | M,R| M,R
購買數(shù)量:
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產品詳情 操作說明 注意事項

Product Information


Description

PABP rabbit polyclonal

Protein full name

Polyadenylate-binding protein 1

Synonyms

PABP-1, Poly(A)-binding protein 1, Pabpc1, Pabp1

Immunogen

Recombinant protein corresponding to Mouse PABP

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Predicted MW.

71 kDa

Observed MW.

71 kDa

Uniprot ID

P29341, Q9EPH8




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

liver, testis, brain

IHC

Mouse, Rat

1: 1000-1: 2500

testis

IF

Mouse, Rat

1: 1000-1: 4000

testis




Background


PABPC1 binds the poly(A) tail of mRNA. It may be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. PABPC1 can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. It may be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.




Images


Western blot analysis of PABP (GB111164) at dilution of 1: 1000
Lane 1: HT29 cell lysate
Lane 2: Hela, cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: Mouse liver tissue lysate
Lane 5: Mouse testis tissue lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat liver tissue lysate
Lane 8: Rat testis tissue lysate
Lane 9: Rat brain tissue lysate

Immunohistochemistry analysis of paraffin-embedded mouse testis using PABP (GB111164) at dilution of 1: 1200

Immunohistochemistry analysis of paraffin-embedded rat testis using PABP (GB111164) at dilution of 1: 1200

Immunofluorescent analysis of paraffin-embedded mouse testis using PABP (GB111164) at dilution of 1: 1200

Immunofluorescent analysis of paraffin-embedded rat testis using PABP (GB111164) at dilution of 1: 1200




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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