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Anti -beta Tubulin Mouse mAb

價(jià)格: 在線咨詢
貨號(hào):
DRF101434
產(chǎn)品規(guī)格:
50 μL
Applications:
WB | IHC | IF
Species:
H,M,R | H,M,R | H,M,R
購(gòu)買數(shù)量:
產(chǎn)品詳情 操作說明 注意事項(xiàng)

Product Information



Description beta Tubulin mouse monoclonal
Protein full name

Tubulin beta chain

Synonyms

TUBB, CDCBM6, M40, OK/SW-cl.56, TUBB1, TUBB5, CSCSC1, tubulin beta class I,Tubulin Beta-5 Chain

Immunogen

KLH conjugated Synthetic peptide corresponding to human β- tubulin

Isotype IgG
Purity Affinity purification

Predicted MW.

55 kDa

Observed MW.

55 kDa
Subcellular location

Cytoplasm

Uniprot ID

P07437,P99024,P69897




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 2000-1: 5000

Brain,lung,Hela,HepG2,A549

IHC

Human, Mouse, Rat

1: 1000-1: 2000

Kidney,liver cancer,esophageal cancer

IF

Human, Mouse, Rat

1: 1000-1: 2000

Kidney,liver cancer,esophageal cancer




Background


Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

         



Images



image.png

Western blot analysis of β- tubulin (GB13017-2) at dilution of 1:2000.


Lane 1:Hela cell lysate

Lane 2:HepG2 cell lysate

Lane 3:A549 cell lysate

Lane 4:CNE2 cell lysate

Lane 5:Jurkat cell lysate

Lane 6:NIH/3T3 cell lysate

Lane 7:Raw264.7 cell lysate

Lane 8:H9C2 cell lysate


image.png

Immunohistochemistry analysis of paraffin-embedded rat brain using β- tubulin (GB13017-2) at dilution of 1:1000

image.png

Immunofluorescence analysis of paraffin-embedded human esophageal cancer using β- tubulin (GB13017-2) at dilution of 1:1000





Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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