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Product Information
Description |
Glutamine Synthetase rabbit polyclonal antibody |
Protein full name |
Glutamine synthetase |
Synonyms |
GS, Glutamate--ammonia ligase, GLUL, Palmitoyltransferase GLUL, GLNS |
Immunogen |
Recombinant protein corresponding to Human Glutamine Synthetase |
Isotype |
IgG |
Purity |
Affinity purification |
Predicted MW. |
42 kDa |
Observed MW. |
42 kDa |
Uniprot ID |
P15105, P09606 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
WB |
Mouse, Rat |
1: 500-1: 1000 |
brain, testis |
Background
Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. It is found in astrocytes as an octamer of identical 42 kDa subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in the metabolic regulation of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer's disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. The level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.
Images
|
Western blot analysis of GluL (GB111217) at dilution of 1: 500 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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