Pre-mRNA branch site protein p14, SF3b 14 kDa subunit, Sf3b6, Sf3b14
Immunogen
KLH conjugated Synthetic peptide corresponding to Mouse SF3B14
Isotype
IgG
Purity
Affinity purification
Subcellular location
Nucleus
Predicted MW.
14 kDa
Observed MW.
14 kDa
Uniprot ID
Q9Y3B4, P59708
Applications
Applications
Species
Dilution
Positive Sample
WB
Human, Mouse, Rat
1:500-1:1000
testis, colon
IHC/IF
Human, Mouse, Rat
1:500-1:2000
brain, colon cancer, heart, liver
Background
This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.
Images
Western blot analysis of SF3B14 (GB111446) at dilution of 1: 500
Immunohistochemistry of paraffin embedded human brain using SF3B14 (GB111446) at dilution of 1: 1000 (200x lens)
Immunohistochemistry of paraffin embedded human colon cancer using SF3B14 (GB111446) at dilution of 1: 1000 (400x lens)
Immunohistochemistry of paraffin embedded mouse liver using SF3B14 (GB111446) at dilution of 1: 1000 (400x lens)
Immunohistochemistry of paraffin embedded rat heart using SF3B14 (GB111446) at dilution of 1: 1000 (300x lens)
Immunofluorescence of paraffin embedded mouse brain using SF3B14 (GB111446) at dilution of 1: 1000 (400x lens)
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer
PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).