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技術(shù)規(guī)格
Background: |
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins. |
Applications: |
ELISA, WB |
Name of antibody: |
FLAG Tag |
Immunogen: |
Synthetic peptide: DYKDDDDK conjugated to KLH |
Full name: |
FLAG Tag |
Synonyms: |
DDDDK epitope tag; DYKDDDDK epitope tag |
ELISA Recommended dilution: |
500-1000 |
WB Predicted band size: |
50 kDa |
WB Positive control: |
Fusion proteins containing the Flag Tag |
WB Recommended dilution: |
10000-50000 |
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