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Background: |
Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. |
Applications: |
WB |
Name of antibody: |
eGFP |
Immunogen: |
eGFP tagged recombinant protein expressed in HEK293T cell |
SwissProt: |
P42212 |
WB Predicted band size: |
27 kDa |
WB Positive control: |
eGFP-fused protein level by using anti-eGFP antibody in HeLa cells after 24 h (Day 1) or 45 h (Day 2); HA-β-globin, co-transfection control EGFP and tubulin (loading control) are indicated. |
WB Recommended dilution: |
1000-5000 |
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