This antibody detects FITC conjunted protein or antibody.
Source
Rabbit
Concentration
1 mg/ml
Purification
The antibody was affinity-purified from rabbit ascites by affinity-chromatography using specific immunogen.
Applications
Applications
Dilution
Elisa
1: 5000-1:10000
Background
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. This rabbit antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody. Moreover, this rabbit antibody used with HRP goat anti-rabbit secondary antibody, converts fluorescent signal to DAB dyeing.
Images
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .