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Anti -Seladin 1 Rabbit pAb

價格: 在線咨詢
貨號:
KT011547
產(chǎn)品規(guī)格:
100 μL
Applications:
WB/IHC/IF
Species:
H/MR
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

Seladin 1 rabbit polyclonal antibody

Protein full name

Delta(24)-sterol reductase

Synonyms

24-dehydrocholesterol reductase, 3-beta-hydroxysterol Delta-24-reductase, Diminuto/dwarf1 homolog, Seladin-1, DHCR24, KIAA0018

Immunogen

Recombinant protein corresponding to Mouse Seladin 1

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW.

60 kDa

Observed MW.

60 kDa

Uniprot ID

Q15392, Q8VCH6, Q5BQE6




Applications


Applications

Species

Dilution

Positive Sample

WB

Human

1: 500-1: 1000

HT-29, HeLa

IHC/IF

Mouse, Rat

1: 500-1: 3000

colon, duodenum, liver, lung, small intestine




Background


The Seladin 1 encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. Protects cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis.Missense mutations in this gene have been associated with desmosterolosis.




Images


Picture 1.png

Western blot analysis of Dhcr24 (GB112463) at dilution of 1: 500

Picture 2.png

Immunohistochemistry of paraffin embedded mouse duodenum using Dhcr24 (GB112463) at dilution of 1: 650 (400x lens)

Picture 3.png

Immunohistochemistry of paraffin embedded mouse liver using Dhcr24 (GB112463) at dilution of 1: 1300 (400x lens)

Picture 4.png

Immunohistochemistry of paraffin embedded mouse lung using Dhcr24 (GB112463) at dilution of 1: 1300 (400x lens)

Picture 5.png

Immunohistochemistry of paraffin embedded rat colon using Dhcr24 (GB112463) at dilution of 1: 650 (400x lens)

Picture 6.png

Immunohistochemistry of paraffin embedded rat liver using Dhcr24 (GB112463) at dilution of 1: 1300 (400x lens)

Picture 7.png

Immunohistochemistry of paraffin embedded rat lung using Dhcr24 (GB112463) at dilution of 1: 1300 (400x lens)

Picture 8.png Immunohistochemistry of paraffin embedded rat small intestine using Dhcr24 (GB112463) at dilution of 1: 650 (400x lens)
Picture 9.png Immunofluorescence of paraffin embedded mouse duodenum using Dhcr24 (GB112463) at dilution of 1: 650 (400x lens)
Picture 10.png Immunofluorescence of paraffin embedded mouse lung using Dhcr24 (GB112463) at dilution of 1: 650 (400x lens)

Picture 11.png

Immunofluorescence of paraffin embedded rat duodenum using Dhcr24 (GB112463) at dilution of 1: 650 (400x lens)

Picture 12.png

Immunofluorescence of paraffin embedded rat lung using Dhcr24 (GB112463) at dilution of 1: 650 (400x lens)




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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