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Product Information
Description |
Vimentin rabbit polyclonal antibody |
Protein full name |
Vimentin |
Synonyms |
VIM, CTRCT30, HEL113, vimentin |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Vimentin |
Isotype |
IgG |
Purity |
Affinity purification |
Predicted MW. |
54 kDa |
Observed MW. |
54 kDa |
Subcellular location |
|
Uniprot ID |
Applications
Applications |
Species |
Dilution |
Positive Sample |
WB |
Human,Mouse,Rat |
1: 500-1: 1000 |
lung |
IHC/IF |
Human,Mouse,Rat |
1: 1500-1: 3000 |
breast cancer, thymus, lymph, colon, lung, kidney, ovary, spleen |
Background
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
Images
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Western blot analysis of Vimentin (GB111308) at dilution of 1: 500 |
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Immunohistochemistry analysis of paraffin-embedded human breast cancer using Vimentin (GB111308) at dilution of 1: 1500 |
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Immunohistochemistry analysis of paraffin-embedded human breast cancer using Vimentin (GB111308) at dilution of 1: 1500 |
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Immunohistochemistry analysis of paraffin-embedded mouse immune spleen using Vimentin (GB111308) at dilution of 1: 1500 |
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Immunohistochemistry analysis of paraffin-embedded mouse placenta using Vimentin (GB111308) at dilution of 1: 3000 |
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Immunohistochemistry analysis of paraffin-embedded rat colon using Vimentin (GB111308) at dilution of 1: 1500 |
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Immunohistochemistry analysis of paraffin-embedded rat lymph using Vimentin (GB111308) at dilution of 1: 3000 |
|
Immunofluorescent analysis of paraformaldehyde-fixed human ovary cancer using Vimentin (GB111308) at dilution of 1: 3000 |
|
Immunofluorescent analysis of paraformaldehyde-fixed human colon using Vimentin (GB111308) at dilution of 1: 3000 |
|
Immunofluorescent analysis of paraformaldehyde-fixed mouse kidney using Vimentin (GB111308) at dilution of 1: 3000 |
|
Immunofluorescent analysis of paraformaldehyde-fixed mouse lung using Vimentin (GB111308) at dilution of 1: 300 |
|
Immunofluorescent analysis of paraformaldehyde-fixed rat ovray using Vimentin (GB111308) at dilution of 1: 3000 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat spleen using Vimentin (GB111308) at dilution of 1: 3000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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