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Product Information
Description |
160 kDa Neurofilament Medium mouse monoclonal antibody |
Protein full name |
Neurofilament medium polypeptide |
Synonyms |
160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, Nef3, Nefm, NF-M, Nfm, 150kDa medium |
Immunogen |
Recombinant protein corresponding to Mouse NF-M |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Cytoplasm, Cytoskeleton |
Predicted MW. |
96 kDa |
Observed MW. |
150 kDa |
Uniprot ID |
P07197, P08553, P12839 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
brain, cerebral cortex, hippocampus, spinal cord |
IHC/IF |
Mouse, Rat |
1: 500-1: 4000 |
brain, spinal cord, epencephalon |
Background
NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Images
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Western blot analysis of NF-M (GB12763) at dilution of 1: 1000
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Immunohistochemistry analysis of paraffin-embedded mouse brain using NF-M (GB12763) at dilution of 1: 4000
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Immunohistochemistry analysis of paraffin-embedded rat brain using NF-M (GB12763) at dilution of 1: 4000 |
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Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using NF-M (GB12763) at dilution of 1: 4000 |
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Immunohistochemistry analysis of paraffin-embedded rat spinal cord using NF-M (GB12763) at dilution of 1: 4000 |
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Immunohistochemistry analysis of paraffin-embedded mouse epencephalon using NF-M (GB12763) at dilution of 1: 4000 | |
Immunohistochemistry analysis of paraffin-embedded rat epencephalon using NF-M (GB12763) at dilution of 1: 4000 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse brain using NF-M (GB12763) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat brain using NF-M (GB12763) at dilution of 1: 1000
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Immunofluorescent analysis of paraformaldehyde-fixed mouse spinal cord using NF-M (GB12763) at dilution of 1: 1000 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat spinal cord using NF-M (GB12763) at dilution of 1: 1000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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