Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, MYH10, non-muscle IIb, NMMHC-B, NMMHC-IIB
Immunogen
KLH conjugated Synthetic peptide corresponding to Mouse MYH10
Isotype
IgG
Purity
Affinity purification
Subcellular location
Nuclear, Cytoplasm, Secreted
Predicted MW.
229 kDa
Observed MW.
260 kDa
Uniprot ID
P35580, Q61879, Q9JLT0
Applications
Applications
Species
Dilution
Positive Sample
WB
Human, Mouse
1: 500-1: 800
brain, kidney
IHC/IF
Mouse, Rat
1: 500-1: 3000
brain, colon, kidney, ovary, small intestine
Background
MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.
Images
Western blot analysis of MYH10 (GB111186) at dilution of 1: 500
Immunohistochemistry of paraffin embedded mouse brain using MYH10 (GB111186) at dilution of 1: 1400 (400x lens)
Immunohistochemistry of paraffin embedded mouse kidney using MYH10 (GB111186) at dilution of 1: 1400 (400x lens)
Immunohistochemistry of paraffin embedded rat brain using MYH10 (GB111186) at dilution of 1: 1400 (400x lens)
Immunohistochemistry of paraffin embedded rat kidney using MYH10 (GB111186) at dilution of 1: 1400 (400x lens)
Immunohistochemistry of paraffin embedded rat ovary using MYH10 (GB111186) at dilution of 1: 1400 (400x lens)
Immunofluorescence of paraffin embedded mouse brain using MYH10 (GB111186) at dilution of 1: 1400 (400x lens)
Immunofluorescence of paraffin embedded mouse small intestine using MYH10 (GB111186) at dilution of 1: 1400 (400x lens)
Immunofluorescence of paraffin embedded rat brain using MYH10 (GB111186) at dilution of 1: 1400 (400x lens)
Immunofluorescence of paraffin embedded rat colon using MYH10 (GB111186) at dilution of 1: 1400 (400x lens)
Immunofluorescence of paraffin embedded rat kidney using MYH10 (GB111186) at dilution of 1: 1400 (400x lens)
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer
PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer.