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Product Information
Description |
Myosin light chain 3 rabbit polyclonal antibody |
Protein full name |
Myosin light chain 3 |
Synonyms |
Myosin alkali light chain 1, ventricular/slow skeletal muscle isoform, MLC1SB, Myl3, Mlc1v, Mylc, Myosin light chain 3 |
Immunogen |
Recombinant protein corresponding to Mouse Myosin light chain 3 |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Cytoplasm |
Predicted MW. |
22 kDa |
Observed MW. |
23 kDa |
Uniprot ID |
P09542, P16409 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
WB |
Mouse, Rat |
1: 500-1: 1000 |
heart, skeletal muscle |
IHC/IF |
Mouse, Rat |
1: 1500-1: 3000 |
esophagus, lung, Skeletal muscle |
Background
MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury.
Images
|
Western blot analysis of MYL3 (GB111241) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded mouse lung using MYL3 (GB111241) at dilution of 1: 3400 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse skeletal muscle using MYL3 (GB111241) at dilution of 1: 1700 (400x lens) |
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Immunohistochemistry of paraffin embedded rat esophagus using MYL3 (GB111241) at dilution of 1: 3400 (400x lens) |
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Immunohistochemistry of paraffin embedded rat skeletal muscle using MYL3 (GB111241) at dilution of 1: 1700 (400x lens) |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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