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Product Information
Description |
Cytokeratin 5 rabbit polyclonal |
Protein full name |
Keratin, type II cytoskeletal 5 |
Synonyms |
Cytokeratin-5, CK-5, Keratin-5, K5, Type-II keratin Kb5, Krt5, Krt2-5 |
Immunogen |
Recombinant protein corresponding to Mouse Cytokeratin 5 |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Cytoplasm, Cell Membrane |
Predicted MW. |
62 kDa |
Observed MW. |
62 kDa |
Uniprot ID |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Mouse |
1: 500-1: 1000 |
skin |
IHC |
Human, Mouse, Rat |
1: 1000-1: 2000 |
breast cancer, skin, bladder |
IF |
Human, Mouse, Rat |
1: 1000-1: 3000 |
ovary, breast, skin, esophagus, bladder |
Background
The protein encoded by this gene is a member of the keratin gene family. It is one type II keratin, is coexpressed with keratin 14 in stratified squamous epithelia. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex.
Images
Western blot analysis of Cytokeratin 5 (GB111246) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded human breast cancer using CK5 (GB111246) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded mouse skin using CK5 (GB111246) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat skin using CK5 (GB111246) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat bladder using CK5 (GB111246) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed human ovary using CK5 (GB111246) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed human breast using CK5 (GB111246) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse skin using CK5 (GB111246) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat esophagus using CK5 (GB111246) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat bladder using CK5 (GB111246) at dilution of 1: 2000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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