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Product Information
Description |
CD34 rabbit polyclonal antibody |
Protein full name |
Hematopoietic progenitor cell antigen CD34 |
Synonyms |
CD34, HPCA1, Mucosialin, Cluster designation 34, cluster of differentiation 34, CD34 antigen |
Immunogen |
Recombinant protein corresponding to Human CD34 |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Membrane |
Predicted MW. |
41 kDa |
Observed MW. |
110 kDa |
Uniprot ID |
P28906, Q64314 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
brain, liver, kidney |
IHC/IF |
Human |
1: 100-1: 1000 |
colon, kidney, placenta |
Background
CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources.
Images
|
Western blot analysis of CD34 (GB111693) at dilution of 1: 500 |
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Western blot analysis of CD34 (GB111693) at dilution of 1: 500 |
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Immunofluorescence of paraffin embedded human colon using CD34 (GB111693) at dilution of 1: 600 (400x lens) |
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Immunofluorescence of paraffin embedded human kidney using CD34 (GB111693) at dilution of 1: 600 (400x lens) |
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Immunofluorescence of paraffin embedded human placenta using CD34 (GB111693) at dilution of 1: 600 (400x lens) |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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