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Product Information
Description |
MCM2 rabbit polyclonal antibody |
Protein full name |
DNA replication licensing factor MCM2 |
Synonyms |
Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, Mcm2, Bm28, Cdcl1, Kiaa0030, Mcmd2 |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse MCM2 |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Nucleus |
Predicted MW. |
102 kDa |
Observed MW. |
130 kDa |
Uniprot ID |
P49736, P97310, D3ZP96 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
thymus |
IHC/IF |
Human, Mouse, Rat |
1: 1000-1: 4000 |
brain, colon cancer, lung, lung cancer, thymus |
Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex.
Images
|
Western blot analysis of MCM2 (GB111162) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded human colon cancer using MCM2 (GB111162) at dilution of 1: 4000 (400x lens) |
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Immunohistochemistry of paraffin embedded human lung cancer using MCM2 (GB111162) at dilution of 1: 4000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse brain using MCM2 (GB111162) at dilution of 1: 2000 (400x lens) |
|
Immunohistochemistry of paraffin embedded mouse thymus using MCM2 (GB111162) at dilution of 1: 4000 (400x lens) |
|
Immunohistochemistry of paraffin embedded rat lung using MCM2 (GB111162) at dilution of 1: 2000 (400x lens) |
|
Immunohistochemistry of paraffin embedded rat thymus using MCM2 (GB111162) at dilution of 1: 4000 (400x lens) |
|
Immunofluorescence of paraffin embedded mouse brain using MCM2 (GB111162) at dilution of 1: 2000 (400x lens) |
|
Immunofluorescence of paraffin embedded rat thymus using MCM2 (GB111162) at dilution of 1: 2000 (400x lens) |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).
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