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Anti -ATG3 Rabbit pAb

價(jià)格: 在線咨詢
貨號:
KT012161
產(chǎn)品規(guī)格:
100 μL
Applications:
WB
Species:
H,M
購買數(shù)量:
產(chǎn)品詳情 操作說明 注意事項(xiàng)

Product Information


Description

ATG3 rabbit polyclonal antibody

Protein full name

Ubiquitin-like-conjugating enzyme ATG3

Synonyms

Autophagy-related protein 3, Protein PC3-96, APG3-like, hApg3, ATG3, APG3, APG3L

Immunogen

Recombinant protein corresponding to Human ATG3

Isotype

IgG

Purity

Affinity purification

Predicted MW.

36 kDa

Observed MW.

40 kDa

Uniprot ID

Q9NT62, Q9CPX6




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse

1: 500-1: 1000

THP-2, A20,

HeLa, Jurkat



Background


Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.




Images


Picture 1.png

Western blot analysis of ATG3 (GB111131) at dilution of 1: 500




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).



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