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Product Information
Description |
beta Actin mouse monoclonal |
Protein full name |
Actin, cytoplasmic 1 |
Synonyms |
ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse β-Actin |
Isotype |
IgG |
Predicted MW. |
42 kDa |
Observed MW. |
45 kDa |
Purity |
Affinity purification |
Subcellular location |
|
Uniprot ID |
P60709, P60710, P60711 |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Rat, Mouse |
1: 1000-1: 2000 |
kidney, brain, spleen |
IHC |
Human, Rat, Mouse |
1: 400-1: 2000 |
para-cancer colon, lung, stomach, liver cancer |
IF |
Human, Rat, Mouse |
1: 400-1: 2000 |
para-cancer colon, lung, stomach, liver cancer, |
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Images
Western blot analysis of β-Actin(GB12001) at dilution of 1: 1000 |
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Western blot analysis of β-Actin(GB12001) at dilution of 1: 1000 |
|
Immunohistochemistry analysis of paraffin-embedded human liver cancer using β-Actin(GB12001) at dilution of 1:400 | |
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Immunohistochemistry analysis of paraffin-embedded human para-cancer colon using β-Actin(GB12001) at dilution of 1:400 |
Immunohistochemistry analysis of paraffin-embedded mouse stomach using β-Actin(GB12001) at dilution of 1:400 |
|
Immunohistochemistry analysis of paraffin-embedded rat liver using β-Actin(GB12001) at dilution of 1:400 |
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Immunohistochemistry analysis of paraffin-embedded rat kidney using β-Actin(GB12001) at dilution of 1:400 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
Publications:
|
Title |
Journal |
Pubmed ID |
Application |
1 |
Time-Restricted Feeding Reduces the Detrimental Effects of a High-Fat Diet, Possibly by Modulating the Circadian Rhythm of Hepatic Lipid Metabolism and Gut Microbiota. |
Frontiers in Nutrition |
33425971 |
WB |
2 |
Linalool inhibits 22Rv1 prostate cancer cell proliferation and induces apoptosis. |
Oncology Letters |
33029205 |
WB |
3 |
TRIM3 inhibits P53 signaling in breast cancer cells. |
Cancer Cell International |
33292295 |
WB |
4 |
CAB39 Promotes the Proliferation of Nasopharyngeal Carcinoma CNE-1 Cells via Up-Regulating p-JNK. |
Cancer Management and Research |
33177871 |
WB |
5 |
MicroRNA23a5p mediates the proliferation and differentiation of C2C12 myoblasts. |
Molecular Medicine Reports |
32901860 |
WB |
6 |
NLRC5 Inhibits Inflammation of Secretory Phase Ectopic Endometrial Stromal Cells by Up-Regulating Autophagy in Ovarian Endometriosis. |
Frontiers in Pharmacology |
33013364 |
WB |
7 |
RACO-1 modulates Hippo signalling in oesophageal squamous cell carcinoma. |
Journal of Cellular and Molecular Medicine |
32896069 |
WB |
8 |
CDE-1 suppresses the production of risiRNA by coupling polyuridylation and degradation of rRNA. |
BMC Biology |
32887607 |
WB |
9 |
Synergistic Effects of Ginkgolide B and Protocatechuic Acid on the Treatment of Parkinson's Disease. |
Molecules (Basel, Switzerland) |
32878312 |
WB |
10 |
Effect of Moxibustion on the Intestinal Flora of Rats with Knee Osteoarthritis Induced by Monosodium Iodoacetate. |
Evidence-based Complementary and Alternative Medicine : eCAM |
32714401 |
WB |
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