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Product Information
Description |
SAE1 mouse monoclonal |
Protein full name |
SUMO-activating enzyme subunit 1 |
Synonyms |
Ubiquitin-like 1-activating enzyme E1A, Sae1, SUMO-activating enzyme subunit 1, Uble1a, Aos1, Sua1, N-terminally processed, Ubl1a1 |
Immunogen |
Recombinant protein corresponding to Mouse SAE1 |
Isotype |
IgG |
Purity |
Affinity purification |
Predicted MW. |
39 kDa |
Observed MW. |
39 kDa |
Uniprot ID |
Q9UBE0, Q9R1T2, Q6AXQ0 |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
testis, colon |
Background
SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Images
|
Western blot analysis of SAE1 at dilution of 1: 1000 Lane 1: Hela cell lysate Lane 2: MCF7 cell lysate Lane 3: 293T cell lysate Lane 4: NIH3T3 cell lysate Lane 5: PC12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Mouse colon tissue lysate Lane 8: Rat testis tissue lysate Lane 9: Rat colon tissue lysate |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer.
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