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Product Information
Description |
NSE mouse monoclonal |
Protein full name |
Gamma-enolase |
Synonyms |
ENO2, HEL-S-279, NSE, Enolase 2 |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse NSE |
Isotype |
IgG2a |
Purity |
Affinity purification |
Subcellular location |
|
Predicted MW. |
47 kDa |
Observed MW. |
47 kDa |
Uniprot ID |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Mouse, Rat |
1: 1000-1: 2000 |
brain, cerebellum, spinal cord |
IHC |
Human, Mouse, Rat |
1: 1000-1: 2000 |
neuroendocrine tumor, brain, cerebellum |
Background
Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene.Gamma-enolase is a phosphopyruvate hydratase.Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival .
Images
|
Western blot analysis of NSE (GB12376) at dilution of 1: 2000 Lane 1: U87 cell lysate Lane 2: U251 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse cerebellum tissue lysate Lane 6: Mouse spinal cord tissue lysate Lane 7: Rat brain tissue lysate Lane 8: Rat cerebellum tissue lysate Lane 9: Rat spinal cord tissue lysate |
|
Immunohistochemistry analysis of paraffin-embedded human neuroendocrine tumor using NSE (GB12376) at dilution of 1: 2000 |
|
Immunohistochemistry analysis of paraffin-embedded mouse brain using NSE (GB12376) at dilution of 1: 2000 |
|
Immunohistochemistry analysis of paraffin-embedded rat brain using NSE (GB12376) at dilution of 1: 2000 |
|
Immunohistochemistry analysis of paraffin-embedded mouse cerebellum using NSE (GB12376) at dilution of 1: 2000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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