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Anti -NSE Mouse mAb

價格: 在線咨詢
貨號:
DRF101402
產品規(guī)格:
100 μL
Applications:
Species:
購買數量:
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產品詳情 操作說明 注意事項

Product Information


Description

NSE mouse monoclonal

Protein full name

Gamma-enolase

Synonyms

ENO2, HEL-S-279, NSE, Enolase 2

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse NSE

Isotype

IgG2a

Purity

Affinity purification

Subcellular location

Cell membrane

Predicted MW.

47 kDa

Observed MW.

47 kDa

Uniprot ID

P09104, P17183 , P07323




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 1000-1: 2000

brain, cerebellum, spinal cord

IHC

Human, Mouse, Rat

1: 1000-1: 2000

neuroendocrine tumor, brain, cerebellum




Background


Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene.Gamma-enolase is a phosphopyruvate hydratase.Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival .




Images


Western blot analysis of NSE (GB12376) 

at dilution of 1: 2000

Lane 1: U87 cell lysate

Lane 2: U251 cell lysate

Lane 3: SH-SY5Y cell lysate

Lane 4: Mouse brain tissue lysate

Lane 5: Mouse cerebellum tissue lysate

Lane 6: Mouse spinal cord tissue lysate

Lane 7: Rat brain tissue lysate

Lane 8: Rat cerebellum tissue lysate

Lane 9: Rat spinal cord tissue lysate

Immunohistochemistry analysis of paraffin-embedded human neuroendocrine tumor using NSE (GB12376) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded mouse brain using 

NSE (GB12376) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded rat brain using 

NSE (GB12376) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded mouse cerebellum using 

NSE (GB12376) at dilution of 1: 2000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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