Anti -160 kDa Neurofilament Medium Mouse mAb

價格：在線咨詢

貨號：

DRF101421

產(chǎn)品規(guī)格：

100 μL

Applications：

Species：

品牌：

萬物

購買數(shù)量：

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產(chǎn)品詳情操作說明注意事項

Product Information

Description	160 kDa Neurofilament Medium mouse monoclonal
Protein full name	Neurofilament medium polypeptide
Synonyms	160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, Nef3, Nefm, NF-M, Nfm, 150kDa medium
Immunogen	Recombinant protein corresponding to Mouse NF-M
Isotype	IgG
Purity	Affinity purification
Subcellular location	Cytoplasm
Predicted MW.	96 kDa
Observed MW.	150 kDa
Uniprot ID	P07197, P08553, P12839

Applications

Applications	Species	Dilution	Positive Tissue
WB	Human, Mouse, Rat	1: 500-1: 1000	brain, cortex, hippocampus, spinal cord
IHC	Mouse, Rat	1: 1000-1: 4000	brain, cerebellum
IF	Mouse, Rat	1: 500-1: 1000

Background

NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber

Images

	Western blot analysis of NF-M (GB12763) at dilution of 1: 1000 Lane 1: Mouse brain tissue lysate Lane 2: Mouse cortex tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Mouse spinal cord tissue lysate Lane 5: Rat brain tissue lysate Lane 6: Rat cortex tissue lysate Lane 7: Rat hippocampus tissue lysate Lane 8: Rat spinal cord tissue lysate
	Immunohistochemistry analysis of paraffin-embedded mouse brain using NF-M (GB12763) at dilution of 1: 4000
	Immunohistochemistry analysis of paraffin-embedded mouse cerebellum using NF-M (GB12763) at dilution of 1: 4000
	Immunohistochemistry analysis of paraffin-embedded rat brain using NF-M (GB12763) at dilution of 1: 4000
	Immunohistochemistry analysis of paraffin-embedded rat cerebellum using NF-M (GB12763) at dilution of 1: 4000

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer	PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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