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Product Information
Description |
Histone H3 mouse monoclonal |
Protein full name |
Histone H3 |
Immunogen |
KLH conjugated Synthetic peptide corresponding to human Histone H3 |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. |
17 kDa |
Observed MW. |
17 kDa |
Uniprot ID |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human,Mouse,Rat,chicken,guinea pig,pig,zebrafish,dog,rabbit |
1:1000-1:3000 |
XXX |
Background
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells.[1][2]Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.
Images
Western blot analysis of Histone H3 (GB13102-1) at dilution of 1:10000. Lane 1: Hela cell lysate Lane 2: HepG2 cell lysate Lane 3: A549 cell lysate Lane 4: HUVEN cell lysate Lane 5: Jurkat cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: RAW264.7 cell lysate Lane 8: H9C2 cell lysate |
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Western blot analysis of Histone H3 (GB13102-1) at dilution of 1:10000. Lane 1:Mouse mixed tissue lysate Lane 2: Rat mixed tissue lysate Lane 3: Chicken mixed tissue lysate Lane 4: Guinea pig mixed tissue lysate Lane 5: Pig mixed tissue lysate Lane 6: zebrafish mixed tissue lysate Lane 7: Dog mixed tissue lysate Lane 8: Rabbit mixed tissue lysate |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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