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Product Information
Description |
CD14 rabbit polyclonal |
Protein full name |
Monocyte differentiation antigen CD14 |
Synonyms |
CD14, LPSR, CD14 molecule |
Immunogen |
Recombinant human CD14 protein expressed in HEK293 cells |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
|
Predicted MW. |
40 kDa |
Observed MW. |
50-60 kDa |
Uniprot ID |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human |
1: 500-1: 1000 |
THP-1, Raji |
IHC |
Human |
1: 500-1: 1000 |
appendix, spleen, tonsil |
IF |
Human |
1: 500-1: 1000 |
tonsil, lung |
Background
CD14 is a 50-55 kDa glycosylphosphatidylinositol anchored glycoprotein. CD14 was preferentially expressed in monocytes and macrophages. CD14 acts as a common receptor of lipopolysaccharide (LPS) (together with TLR4 and MD-2). It plays an important role in the inflammatory response of monocytes to LPS.
Images
|
Western blot analysis of CD14 (GB113374) at dilution of 1: 1000 Lane1: THP-1 cell lysate Lane2: Raji cell lysate Lane3-6: Recombinant antigen protein lysate in different dilution gradient |
|
Immunohistochemistry analysis of paraffin-embedded human appendix using CD14 (GB113374) at dilution of 1: 1000 |
|
Immunohistochemistry analysis of paraffin-embedded human spleen using CD14 (GB113374) at dilution of 1: 1000 |
|
Immunohistochemistry analysis of paraffin-embedded human tonsil using CD14 (GB113374) at dilution of 1: 1000 |
|
Immunofluorescent analysis of paraformaldehyde-fixed human lung using CD14 (GB113374) at dilution of 1: 1000 |
|
Immunofluorescent analysis of paraformaldehyde-fixed human tonsil using CD14 (GB113374) at dilution of 1: 1000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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