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Anti -CD20 Rabbit pAb

價格: 在線咨詢
貨號:
DRF101870
產(chǎn)品規(guī)格:
100 μL
Applications:
Species:
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

CD20 rabbit polyclonal

Protein full name

B-lymphocyte antigen CD20

Synonyms

MS4A1, B1, Bp35, CD20, CVID5, LEU-16,

MS4A2, S7, membrane spanning 4-domains A1

Immunogen

Recombinant protein corresponding to Mouse CD20

Isotype

IgG

Purity

Affinity purification

Predicted MW.

32 KDa

Observed MW.

37 KDa

Subcellular location

Membrane

Uniprot ID

P19437




Applications


applications

species

dilution

Positive tissue

WB

Mouse, Rat

1: 500-1: 1000

spleen, lymph gland, thymus

IHC

Mouse, Rat

1: 500-1: 3000

spleen

IF

Mouse, Rat

1: 500-1: 1000

lymph, Inflammatory spleen, spleen




Background


B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. The protein has no known natural ligand[8] and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that it acts as a calcium channel in the cell membrane. It has been shown that CD20 plays a role in the microenvironmental interactions of B cells.




Images



1555379385123367.png

Western blot analysis of CD20 (GB11540) at dilution of 1:1000.

Lane 1:A20 cell lysate

Lane 2:Mouse inflammatory spleen tissue lysate

Lane 3:Mouse spleen tissue lysate

Lane 4:Mouse lymph gland tissue lysate

Lane 5:Rat inflammatory spleen tissue lysate

Lane 6:Rat spleen tissue lysate

Lane 7:Rat lymph gland tissue lysate

Lane 8:Rat thymus tissue lysate

image.png

Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD20 (GB11540) at dilution of 1:500

image.png

Immunohistochemistry analysis of paraffin-embedded rat spleen using CD20 (GB11540) at dilution of 1:1000

Immunofluorescent analysis of paraformaldehyde-fixed mouse lymph using CD20 (GB11540) at dilution of 1:1000

Immunofluorescent analysis of paraformaldehyde-fixed mouse Inflammatory spleen using CD20 (GB11540) at dilution of 1:1000

Immunofluorescent analysis of paraformaldehyde-fixed rat spleen using CD20 (GB11540) at dilution of 1:1000




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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