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Product Information
Description |
Calbindin rabbit polyclonal antibody |
Protein full name |
Calbindin |
Synonyms |
Calbindin D28, D-28K, PCD-29, Spot 35 protein, Vitamin D-dependent calcium-binding protein, avian-type, Calb1 |
Immunogen |
Recombinant protein corresponding to Mouse Calbindin |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Nucleus, Cytoplasm |
Predicted MW. |
30 kDa |
Observed MW. |
30 kDa |
Uniprot ID |
P05937, P12658, P07171 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
WB |
Mouse, Rat |
1: 500-1: 1000 |
brain, kidney, pituitary |
IHC/IF |
Human, Mouse, Rat |
1: 1000-1: 3000 |
brain, kidney, renal cancer, small intestine |
Background
Calbindin is a member of the calcium-binding protein super family that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.
Images
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Western blot analysis of CALB1 (GB111211) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded human renal cancer using CALB1 (GB111211) at dilution of 1: 1000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse brain using CALB1 (GB111211) at dilution of 1: 2000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse kidney using CALB1 (GB111211) at dilution of 1: 1000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat brain using CALB1 (GB111211) at dilution of 1: 2000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat kidney using CALB1 (GB111211) at dilution of 1: 2000 (400x lens) |
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Immunofluorescence of paraffin embedded mouse kidney using CALB1 (GB111211) at dilution of 1: 2000 (400x lens) |
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Immunofluorescence of paraffin embedded mouse small intestine using CALB1 (GB111211) at dilution of 1: 2000 (400x lens) |
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Immunofluorescence of paraffin embedded rat brain using CALB1 (GB111211) at dilution of 1: 2000 (400x lens) |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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