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Product Information
Description |
NeuN rabbit polyclonal antibody |
Protein full name |
RNA binding protein fox-1 homolog 3 |
Synonyms |
FOX3, HRNBP3, NeuN, RBFOX3 |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse NeuN |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Nucleus, Cytoplasm |
Predicted MW. |
41 kDa |
Observed MW. |
48 kDa |
Uniprot ID |
Q8BIF2 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
WB |
Mouse, Rat |
1:500-1:1000 |
brain, cortex, hippocampus |
IHC/IF |
Mouse, Rat |
1:500-1:1000 |
brain, hippocampus |
Background
NeuN, encoded by FOX3, is a neuron-specific nuclear protein. Anti-NeuN stains exclusively neuronal cells in the central and peripheral nervous systems, especially postmitotic and differentiating neurons, as well as terminally differentiated neurons. Anti-NeuN has been used widely as a reliable tool to detect most postmitotic neuronal cell types. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm.
Images
|
Western blot analysis of Neun (GB11138) at dilution of 1: 500 |
|
Immunohistochemistry of paraffin embedded rat brain using neun (GB11138) at dilution of 1: 1000 (200x lens) |
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Immunohistochemistry of paraffin embedded mouse brain using neun (GB11138) at dilution of 1: 500 (300x lens)
|
Immunofluorescence of paraffin embedded rat hippocampus using neun (GB11138) at dilution of 1: 500 (200x lens) |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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