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Product Information
Description |
Myelin Basic Protein rabbit polyclonal antibody |
Protein full name |
Myelin Basic Protein |
Synonyms |
MBP, entrez:4155, myelin basic protein |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Myelin Basic Protein |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Myelin membrane, Cytoplasmic side of myelin |
Predicted MW. |
27 kDa |
Observed MW. |
17/20 kDa |
Uniprot ID |
P04370, P02688 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
WB |
Mouse, Rat |
1:500-1:1000 |
brain |
IHC/IF |
Mouse, Rat |
1:300-1:600 |
brain |
Background
The classic group of MBP isoforms (isoform 4-isoform 13) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function.
Images
|
Western blot analysis of Myelin Basic Protein (GB11226) at dilution of 1: 500 |
|
Immunofluorescence of paraffin embedded mouse brain using MBP (GB11226) at dilution of 1: 300 (400x lens) |
|
Immunofluorescence of paraffin embedded rat brain using MBP (GB11226) at dilution of 1: 300 (400x lens) |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer.
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