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Anti -PCNA Rabbit pAb

價格: 在線咨詢
貨號:
KT011631
產(chǎn)品規(guī)格:
100 μL
Applications:
WB | IHC | IF
Species:
H,M/H,M
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項(xiàng)

Product Information


Description

PCNA rabbit polyclonal antibody

Protein full name

Proliferating cell nuclear antigen

Synonyms

PCNA, ATLD2, proliferating cell nuclear antigen

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse PCNA

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW.

29 kDa

Observed MW.

36 kDa

Uniprot ID

P12004, P17918, P04961



Applications


Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1: 1000-1: 2000

HeLa, SW480, B16, C6, 7721, HCT116

IHC/IF

Human, Mouse, Rat

1: 200-1: 1000

intestinal cancer, liver cancer, lung cancer, ovarian cancer, testis



Background


Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.




Images



Picture 1.png

Western blot analysis of PCNA (GB11010) at dilution of 1: 1000


Picture 2.png

Western blot analysis of PCNA (GB11010) at dilution of 1: 1000

Picture 3.png

Immunohistochemistry of paraffin embedded human liver cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens)

Picture 4.png

Immunohistochemistry of paraffin embedded human lung cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens)

Picture 5.png

Immunofluorescence of paraffin embedded human intestinal cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens)


Picture 6.png

Immunofluorescence of paraffin embedded human intestinal cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens)


Picture 7.png

Immunofluorescence of paraffin embedded rat testis using PCNA (GB11010) at dilution of 1: 500 (200x lens)


Immunofluorescence of paraffin embedded mouse testis using PCNA (GB11010) at dilution of 1: 500 (200x lens)





Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .



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