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Product Information
Description |
PGP9.5 rabbit polyclonal |
Protein full name |
Ubiquitin carboxyl-terminal hydrolase isozyme L1 |
Synonyms |
UCHL1, HEL-117, NDGOA, PARK5, PGP 9.5, PGP9.5, PGP95, Uch-L1, HEL-S-53, SPG79 |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse PGP9.5 |
Isotype |
IgG |
Purity |
Affinity purification |
Predicted MW. |
27 kDa |
Observed MW. |
27 kDa |
Subcellular location |
Cytoplasm,Endoplasmic reticulum membrane |
Uniprot ID |
Applications
applications |
species |
dilution |
Positive tissue |
WB |
Human, Mouse, Rat |
1: 500-1: 1000 |
brain, cerebellum, spinal marrow |
IHC |
Mouse, Rat |
1: 1000-1: 2000 |
brain |
IF |
Mouse, Rat |
1: 1000-1: 2000 |
brain |
Background
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity (By similarity).
Images
|
Western blot analysis of PGP9.5 (GB11159-1) at dilution of 1:600. Lane 1: Mouse brain tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Mouse cerebral cortex tissue lysate Lane 4: Rat brain tissue lysate Lane 5: Rat cerebellum tissue lysate Lane 6: Rat cerebral cortex tissue lysate |
|
Immunohistochemistry analysis of paraffin-embedded mouse brain using PGP9.5 (GB11159-1) at dilution of 1:1000 |
|
Immunohistochemistry analysis of paraffin-embedded rat brain using PGP9.5 (GB11159-1) at dilution of 1:1000 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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