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Product Information
Description |
Ki67 rabbit polyclonal antibody |
Protein full name |
Proliferation marker protein Ki-67 |
Synonyms |
MKI67, marker of proliferation Ki-67, KIA, MIB-1, PPP1R105 |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Ki67 |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Nucleus |
Uniprot ID |
E9PVX6 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
IHC/IF |
Mouse, Rat |
1: 500-1: 1200 |
colon, placenta, subcutaneous transplant tumor(H22), testis |
Background
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226).
Images
Immunohistochemistry of paraffin embedded mouse placenta using ki67 (GB111141) at dilution of 1:1000 (300x lens) |
|
Immunohistochemistry of paraffin embedded mouse subcutaneous transplant tumor(H22) using Ki67 (GB111141) at dilution of 1:500 (400x lens) | |
Immunohistochemistry of paraffin embedded rat colon using ki67 (GB111141) at dilution of 1:750 (400x lens) |
|
Immunofluorescence of paraffin embedded mouse testis using ki67 (GB111141) at dilution of 1:600 (200x lens) | |
Immunofluorescence of paraffin embedded rat colon using ki67 (GB111141) at dilution of 1:600 (200x lens) |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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