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Anti -GAD65 Mouse mAb

價(jià)格: 在線咨詢
貨號(hào):
KT011779
產(chǎn)品規(guī)格:
100 μL
Applications:
WB/IHC/IF
Species:
H/M/R,M/R,M/R
購買數(shù)量:
產(chǎn)品詳情 操作說明 注意事項(xiàng)

Product Information


Description

GAD65 mouse monoclonal antibody

Protein full name

Glutamate decarboxylase 2

Synonyms

GAD2, GAD65, glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, DCE 2, Glutamic Acid Decarboxylase 2; Glutamic Acid Decarboxylase 65

Immunogen

Recombinant protein corresponding to Mouse GAD65

Isotype

IgG

Purity

Affinity purification

Predicted MW.

65 kDa

Observed MW.

65 kDa

Uniprot ID

Q05329, Q05683, P48320




Applications


Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1:500-1:1000

brain, cerebral cortex, hippocampus, cerebellum

IHC/IF

Mouse, Rat

1:500-1:2000

brain, cerebellum, epencephalon




Background


This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.




Images



image.png

Western blot analysis of GAD65 (GB12562) at dilution of 1: 1000

image.png

Immunohistochemistry of paraffin embedded mouse cerebellum using GAD65 (GB12562) at dilution of  (300x lens)

image.png

Immunohistochemistry analysis of paraffin-embedded mouse brain using GAD65 (GB12562) at dilution of 1: 1000(350x lens)




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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