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Product Information
Description |
Ki67 rabbit polyclonal antibody |
Protein full name |
Proliferation marker protein Ki-67 |
Synonyms |
MKI67, marker of proliferation Ki-67, KIA, MIB-1, PPP1R105 |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Human Ki67 |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Nucleus |
Uniprot ID |
P46013, E9PVX6 |
Applications
Applications |
Species |
Dilution |
Positive Sample |
IHC/IF |
Human, Mouse, Rat |
1: 400-1: 1000 |
colon cancer, esophagus, esophagus cancer, intestinal cancer, ovary, small intestine, spleen |
Background
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226).
Images
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Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Ki67 (GB111499) at dilution of 1: 1200 |
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Immunohistochemistry analysis of paraffin-embedded human colon cancer using Ki67 (GB111499) at dilution of 1: 1200 |
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Immunohistochemistry analysis of paraffin-embedded mouse ovary using Ki67 (GB111499) at dilution of 1: 1200
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Immunohistochemistry analysis of paraffin-embedded rat small intestine using Ki67 (GB111499) at dilution of 1: 1200
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Immunofluorescent analysis of paraformaldehyde-fixed human small intestine cancer using Ki67 (GB111499) at dilution of 1: 1200
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Immunofluorescent analysis of paraformaldehyde-fixed human esophagus using Ki67 (GB111499) at dilution of 1: 1200 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat spleen using Ki67 (GB111499) at dilution of 1: 1200
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Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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