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Product Information
Description | Rad6 rabbit polyclonal |
Protein full name | Ubiquitin-conjugating enzyme E2 A |
Synonyms | E2 ubiquitin-conjugating enzyme A, RAD6 homolog A, HR6A, mHR6A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A, Ube2a, Rad6a |
Immunogen | Recombinant protein corresponding to Mouse Rad6 |
Isotype | IgG |
Purity | Affinity purification |
Predicted MW. | 17 kDa |
Observed MW. | 17 kDa |
Uniprot ID | P49459, Q9Z255 |
Applications
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | placenta, brain, kidney |
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. It plays a critical role in regulating TP53 protein levels under both normal and stress conditions.
Images
Western blot analysis of Rad6 (GB111539) at dilution of 1: 1000 Lane 1: Hela cell lysate Lane 2: A549 cell lysate Lane 3: HEK293 cell lysate Lane 4: Mouse placenta tissue lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse kidney tissue lysate Lane 7: Rat placenta tissue lysate Lane 8: Rat brain tissue lysate Lane 9: Rat kidney tissue lysate |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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