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Product Information
Description |
Cdk2 rabbit polyclonal |
Protein full name |
Cyclin-dependent kinase 2 |
Synonyms |
Cell division protein kinase 2, p33 protein kinase, CDK2, CDKN2, CDC28, CDC2A, |
Immunogen |
KLH conjugated Synthetic peptide corresponding to Human Cdk2 |
Isotype |
IgG |
Purity |
Affinity purification |
Subcellular location |
Nucleus, Cytoplasm |
Predicted MW. |
34 kDa |
Observed MW. |
34 kDa |
Uniprot ID |
P24941, P97377, Q63699 |
Applications
Applications |
Species |
Dilution |
Positive Tissue |
WB |
Human, Mouse, Rat |
1: 400-1: 800 |
spleen, thymus, lymph nodes |
IHC |
Human, Mouse, Rat |
1: 200-1: 800 |
B-lymphoma, spleen, thymus |
Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B).
Images
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Western blot analysis of Cdk2 at dilution of 1: 800 Lane 1: HeLa cell lysate Lane 2: K562 cell lysate Lane 3: MCF7 cell lysate Lane 4: Mouse spleen tissue lysate Lane 5: Mouse thymus tissue lysate Lane 6: Mouse lymph nodes tissue lysate Lane 7: Rat thymus tissue lysate Lane 8: Rat lymph nodes tissue lysate |
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Immunohistochemistry analysis of paraffin-embedded human B-lymphoma using Cdk2 at dilution of 1: 400 |
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Immunohistochemistry analysis of paraffin-embedded mouse spleen using Cdk2 at dilution of 1: 400 |
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Immunohistochemistry analysis of paraffin-embedded rat spleen using Cdk2 at dilution of 1: 400 |
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Immunohistochemistry analysis of paraffin-embedded mouse thymus using Cdk2 at dilution of 1: 400 |
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Immunohistochemistry analysis of paraffin-embedded rat thymus using Cdk2 at dilution of 1: 400 |
Storage
Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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