KLH conjugated Synthetic peptide corresponding to Mouse SAE1
Isotype
IgG
Purity
Affinity purification
Subcellular location
Nucleus
Predicted MW.
39 kDa
Observed MW.
39 kDa
Uniprot ID
Q9UBE0, Q9R1T2, Q6AXQ0
Applications
Applications
Species
Dilution
Positive Sample
WB
Human, Mouse, Rat
1: 500-1: 1000
testis
IHC/IF
Human, Mouse, Rat
1: 750-1: 3000
brain, breast cancer, colon, colon cancer, lung, small intestine, testis
Background
SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Images
Western blot analysis of SAE1 (GB111191) at dilution of 1: 500
Immunohistochemistry of paraffin embedded human breast cancer using SAE1 (GB111191) at dilution of 1: 1200 (400x lens)
Immunohistochemistry of paraffin embedded human colon cancer using SAE1 (GB111191) at dilution of 1: 1200 (400x lens)
Immunohistochemistry of paraffin embedded human colon using SAE1 (GB111191) at dilution of 1: 1200 (400x lens)
Immunohistochemistry of paraffin embedded mouse brain using SAE1 (GB111191) at dilution of 1: 1200 (400x lens)
Immunohistochemistry of paraffin embedded mouse small intestine using SAE1 (GB111191) at dilution of 1: 1200 (400x lens)
Immunohistochemistry of paraffin embedded rat brain using SAE1 (GB111191) at dilution of 1: 1200 (400x lens)
Immunohistochemistry of paraffin embedded rat colon using SAE1 (GB111191) at dilution of 1: 1200 (400x lens)
Immunofluorescence of paraffin embedded human breast cancer using SAE1 (GB111191) at dilution of 1: 1100 (400x lens)
Immunofluorescence of paraffin embedded mouse brain using SAE1 (GB111191) at dilution of 1: 1100 (400x lens)
Immunofluorescence of paraffin embedded mouse lung using SAE1 (GB111191) at dilution of 1: 1100 (400x lens)
Immunofluorescence of paraffin embedded rat brain using SAE1 (GB111191) at dilution of 1: 1100 (400x lens)
Immunofluorescence of paraffin embedded rat testis using SAE1 (GB111191) at dilution of 1: 1100 (300x lens)
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer
PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .