Brca2, BRCA2, BRCC2, BROVCA2, FACD, FAD, FAD1, FANCD, FANCD1, GLM3, PNCA2, XRCC11, Breast cancer 2, DNA repair associated, breast cancer 2, Early onset, BRCA2 DNA repair associated
Immunogen
Recombinant protein corresponding to Mouse BRCA2
Isotype
IgG
Purity
Affinity purification
Subcellular location
Cytoplasm, Nucleus
Uniprot ID
P97929, O35923
Applications
Applications
Species
Dilution
Positive Sample
IHC/IF
Mouse, Rat
1:1500-1:3000
brain, hippocampus
Background
BRCA2, also named as FACD and FANCD1, is involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. May participate in S phase checkpoint activation.
Images
Immunohistochemistry of paraffin embedded mouse hippocampus using BRCA2 (GB111138) at dilution of 1:2200 (400x lens)
Immunohistochemistry of paraffin embedded rat hippocampus using BRCA2 (GB111138) at dilution of 1:1100 (400x lens)
Immunofluorescence of paraffin embedded mouse brain using BRCA2 (GB111138) at dilution of 1:2200 (400x lens)
Immunofluorescence of paraffin embedded rat brain using BRCA2 (GB111138) at dilution of 1:2200 (400x lens)
Immunofluorescence of paraffin embedded rat hippocampus using BRCA2 (GB111138) at dilution of 1:2200 (400x lens)
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer
PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).